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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential part in advertising mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts just isn't but available.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guide outlines further more attainable optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This information outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope on the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Goal Facts Cyclin E belongs to your hugely conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which contribute to your temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S more info section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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